FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4581932
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG229540.500967714055992No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGA55310.12071327117032728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT54850.038.987561
TGGTATC56650.037.2430082
GTATCAA215000.029.3142451
TATCAAC244850.025.6705652
CGACGAG2250.025.66655524
AGAGTAC123800.023.9899411
CAGAGTA130650.023.21625310
ATCAACG269650.023.109993
GGTATCA99250.023.0991571
CAACGCA270950.022.9773065
GAACCGT2300.022.7165496
TCAACGC280350.022.197084
AACGCAG293650.021.4541666
GAGTACG136850.021.19989612
ACGAGTC2600.021.15352226
GTGCCGT956.0653718E-520.26284826
AGTACGG143200.020.16357813
GCGGTAT1651.9481377E-920.0160771
ATGCGAC3350.019.70140621
CGCAGAG320550.019.3110458
Produced by FastQC (version 0.11.5)