FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1719593
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38820.22575109342734007No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30570.1777746245768621No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT29880.17376204718209481No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT21820.12689049094756724No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA21410.12450620582893743No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19250.11194509398444864No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18410.10706021715603635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT24700.047.6740461
TGGTATC25900.045.9455832
AGCGTCA3650.044.2462233
GTATCAA100550.042.531931
GCGTCAG3800.042.4996644
TTAGTCG450.001663340337.777484
GTCGACA709.234793E-636.437821
TCGACTT10300.036.3103942
TATCAAC119700.036.144242
GCAGCGT4950.032.6345441
CAACGCA135400.032.26715
GGTATCA51400.032.2552721
ATCAACG135600.032.219513
TCAACGC138500.031.6676244
CGACTTT11900.031.0711863
GTACGCA550.00444309430.9169391
CGTCAGA5300.030.4714585
GTCGACT12800.029.8903981
AACGCAG145950.029.819936
CGCAGAG160100.026.9454548
Produced by FastQC (version 0.11.5)