FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1110909
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG223482.0116859256698794No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA19550.17598201112782413No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAAC18260.1643698988846071No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAA17220.15500819599085072No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTT17070.15365795038117433No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGA16580.14924714805623143No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTAC13670.12305238322850927No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCA13260.11936171189539377No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAAT13110.11801146628571738No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCT11700.10531915755475922No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCA11410.10270868270938485No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT24850.042.513411
TGGTATC28750.036.82882
CGCAGCT300.001926175436.6644178
AGTCCTA2650.035.2824429
ACGTGCC558.703755E-734.9978528
TACTAGG2850.033.7744182
GCATCGT2850.032.8050048
CAATGCG2850.031.84301419
CTAGGCA3050.031.55264
ATCGTCA3150.031.42664110
GTATCAA119000.030.5867981
GCCATGG453.7978328E-430.55230527
CGGGACG553.49435E-529.99545917
CTAGCAC653.205978E-629.6148993
GTACTAG3450.029.5055521
GTATCAG400.00785637127.5119341
CGTCGAC400.00786846427.50326355
GCATTAG400.00787365227.4995550
TCAATAC507.0324424E-427.499553
GGGTCGT400.007877110527.49707451
Produced by FastQC (version 0.11.5)