FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4048128
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65750.16242075349396065No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48300.1193144090305445No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT47190.11657240087269967No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG1100.054.092389
GTGGTAT39950.041.8122371
GTATCAA166200.039.7162361
TGGTATC44900.037.6731722
TATCAAC181900.036.7996182
AGCGTCA9250.035.3789063
ATCAACG202250.033.3911063
CAACGCA202900.033.2631875
TCAACGC208200.032.4368444
GGTATCA80600.032.220611
GCGTCAG10250.031.9273074
AACGCAG223950.030.2702336
CGCAGAG250650.026.7741228
ACGCAGA259200.026.0221237
CGACTTT17200.025.9451583
GCAGCGT13250.025.6626631
CGTCAGA12850.025.4673085
GCAGAGT266000.025.2130959
TCGAGGG52200.024.9144739
GTCGACT20750.022.327291
Produced by FastQC (version 0.11.5)