FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2926076
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83190.28430567080280894No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63380.2166040800033902No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT56970.19469760867455255No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41900.14319518700129458No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA40360.1379321658084069No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT38670.13215651268114703No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC33590.114795377837076No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT41150.048.654231
TGGTATC43650.046.6395072
GTATCAA169650.043.347271
CGTCAGA6250.037.4018255
AGCGTCA5950.037.144043
TATCAAC200900.037.0643962
GGTATCA84050.035.2504431
ATCAACG223450.033.5141533
CAACGCA228350.032.870015
TCAACGC227600.032.8470424
AACGCAG240750.031.2503036
GTCGACA1701.2732926E-1130.0055681
CTGTGCG7950.029.4054589
TCGACTT15650.029.3300042
CGACTTT16050.028.8638463
GCATCGT3900.028.3361728
GCGTCAG8100.028.3342344
CGCAGAG275850.027.0264518
TCGAGGG62350.026.9273159
GCAGAGT281650.026.5906269
Produced by FastQC (version 0.11.5)