FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803463
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG201871.119346501702558No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA20890.1158327062989371No Hit
GTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTTCAGTACCATTG20440.11333750678555646No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAA18310.10152689575555474No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTAG1150.043.072751
GTGGTAT42250.041.3594671
TGGTATC43800.039.873712
GGGGTAC701.6099511E-731.4281418
TGCTTAA3450.031.08739719
GTATCAA194050.030.3053171
CGTTGTG606.31435E-527.5034392
CGTTCGG904.7220965E-827.50038724
GATGTAC400.00787482527.4996268
TACCTTG400.00787482527.4996267
CGCTTTA507.034044E-427.49962444
TCGACGA904.724643E-827.49886115
AGAGTAC124200.027.03465511
CAGAGTA127050.026.70959310
TCGTTCG957.9760866E-826.05299823
TATCAAC227450.025.9375152
TAACGCA858.797015E-725.88271723
GCTTAAC858.797015E-725.88271720
ATCGGTC651.08940076E-425.3842714
GGTATCA86150.024.979251
Produced by FastQC (version 0.11.5)