FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1885035
Sequences flagged as poor quality0
Sequence length61
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110920.5884240876164103No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGA27930.14816701016161504No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGG19610.1040298986490967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT27550.040.4480131
TGGTATC28600.038.8544542
TGCTACG701.6101694E-731.42778419
CGCTCGA901.4006218E-930.555622
CAATGCG852.7117494E-829.11691719
GTATCAA117150.028.7711751
GCTACGC805.182337E-727.5000420
ACGAGTC1004.3201E-927.5000426
TGCGACG904.723006E-827.50003822
AGAGTAC64500.025.87919611
CGCCAGC1950.025.38465334
TATCAAC133100.025.0053422
CAGAGTA68300.024.72119510
TTAAACG3900.024.67952322
GGTATCA53750.023.8545531
GTCGTCT2000.023.37565453
CGGGCCG1653.6379788E-1223.33274817
GAACCGT952.3177981E-623.1573166
AGCTCGT1950.022.56353838
ATCAACG147100.022.4705433
Produced by FastQC (version 0.11.5)