FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006176
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43250.4298452755780301No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT34300.3408946347358712No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33860.33652164233692716No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT23480.2333587761981999No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23030.22888639760837068No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA22390.2225256813917247No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22310.22173059186464394No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC18820.18704481124574626No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA18780.1866472664822059No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC16570.1646829182966002No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC14590.14500445250135166No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC14580.14490506631046654No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13350.13268056483160004No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT12950.1287051171961963No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC12810.12731371052380497No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT12630.1255247590878733No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC12540.12463028336990745No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11620.11548675380847882No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAA11010.10942419616448813No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC10800.10733708615590115No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10560.1049518175746589No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10310.10246716280253156No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT21550.053.2561041
TGGTATC23900.049.435062
GTATCAA99000.043.5796551
TAGGCAT2950.040.3389135
TATCAAC116250.037.9117622
TCTAACG903.1908712E-837.777712
CGACTTT8900.037.7246553
GGTATCA46050.037.014141
TAGTACT4600.036.0325434
TCGACTT9400.035.718022
CATCTAG601.594007E-435.4218831
TAGTACG1202.8740033E-1035.4218831
TACGCAG554.809408E-934.77266722-23
GTCTAAC1008.0535756E-834.005011
AGGGTAC751.4835199E-533.9999434
CTGTGCG5000.033.999949
ATCAACG131700.033.6578753
TATTCTC4300.033.6045955
ATTCTCT4600.033.260816
GCATCGT2850.032.8069578
Produced by FastQC (version 0.11.5)