FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1725896
Sequences flagged as poor quality0
Sequence length61
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG92600.5365329081242438No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT20900.039.4914321
CGACCGT358.670256E-539.28515219
ACGAGTC1700.038.82297526
CGACGAG1650.038.33278724
TGGTATC21800.036.961552
GCATCGT1950.035.255918
GCGACGA1800.035.1383923
CGAGTCC2000.034.37351627
TGCGACG1950.033.84567322
CGTATCG601.7180646E-632.08287413
CTGGTCG2400.032.08287450
TGCACGG453.7966447E-430.55512212
GTATCAA109100.030.4880351
AGGTCGT2950.028.89789222
GTCAGCG2600.028.55811343
CGCTGGT2750.028.00041248
CATTGCG2950.027.96489529
ATACGTC400.00787586827.4988126
TATCAAC122800.026.7837832
TCAGCGC2900.026.55211644
Produced by FastQC (version 0.11.5)