FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1632380
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33220.2035065364682243No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA26720.16368737671375538No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24970.15296683370293682No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT23610.1446354402773864No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT19020.11651698746615372No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18730.11474044033864665No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA18030.11045222313431921No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17910.10971710018500595No Hit
CTGTAGTCCTTTCTCTCAATTTTCTTTTAAATACATTTTTTACTCCATGA17840.1092882784645732No Hit
CAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATGCGTTAACTAGGC16520.10120192602212719No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA2850.049.213243
GTGGTAT18550.047.4357531
GTATCAA84200.042.6603131
TGGTATC21450.041.0162472
TCGACTT7850.038.983042
GGTATCA40750.037.97111
CGACTTT8200.036.800813
TATCAAC103750.035.5176242
GTCGACT9000.034.47941
GCGTCAG4250.034.0018774
GCATCGT751.484128E-533.9997948
ATCAACG110700.033.2877433
TCAACGC114050.032.1609154
CAACGCA114300.032.01625
CGTCAGA5000.028.9015925
AACGCAG129100.028.4117136
CGCAGAG134850.026.883418
GCAGCGT5600.025.8089491
GCAGAGT141150.025.7437299
CTGTGCG2351.8189894E-1225.3189959
Produced by FastQC (version 0.11.5)