FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1804568
Sequences flagged as poor quality0
Sequence length91
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35870.19877333522482946No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA33500.18563999804939466No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28240.15649174760940016No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT24170.13393787321951847No Hit
CTGTAGTCCTTTCTCTCAATTTTCTTTTAAATACATTTTTTACTCCATGA19840.10994321078507434No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT19610.10866866751488445No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19330.10711704962074026No Hit
CTTCTATCTTCTCCAAATTGTTTCATCCTACCACTCCCAATTAATCTTTC18690.10357049443412494No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT19750.047.7836151
TGGTATC21700.043.871772
GTATCAA86850.043.8072851
AGCGTCA4050.038.8278663
TATCAAC102450.037.7508132
GCAGCGT4300.037.5671541
ATCAACG112550.034.6274643
CAACGCA114550.034.0228845
GGTATCA41900.033.5820431
TCAACGC116400.033.5186544
CTGTGCG2450.031.225069
CGACTTT9250.030.7843483
AACGCAG137400.028.6431716
CGCAGAG138550.028.0066478
GCGTCAG6000.027.6255044
TCGACTT10700.027.009842
GCAGAGT144700.026.9631759
GTCGACT10500.026.3158381
GTACTAG2300.025.8757741
CGTGCAA1658.6747605E-925.7580458
Produced by FastQC (version 0.11.5)