FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1804568
Sequences flagged as poor quality0
Sequence length61
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG192241.065296514179571No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGA29590.1639727624561668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT23450.040.9594731
TGGTATC25550.037.570022
CGTAGGG300.001925609136.6678555
GTATCAA104850.030.5269261
GTCCGTT400.007844361527.5214791
TATCAAC119500.026.5149882
GTCGGTA858.7977423E-725.8824726
CGACTTT6600.025.8327353
ATGCGAC1409.094947E-1225.53582821
CAATGCG1409.094947E-1225.53582819
AGAGTAC61950.024.94695911
TGCGACG1351.6007107E-1024.44455322
CAGAGTA64050.024.34369910
ATCAACG134550.023.5854823
CAACGCA135550.023.1870385
TCAACGC137050.022.9934484
TCGACTT7550.022.9509412
CAGGTCG600.002036907722.91486539
GGTATCA45600.022.451731
CGAGGGG12950.022.08442910
Produced by FastQC (version 0.11.5)