FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1572615
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG143460.9122385326351332No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTA150.005782894355.0026720
GTGGTAT10800.032.3673251
GTATCAA43600.027.9668831
GGCGCTA507.033697E-427.49958610
CGTCGGC759.751739E-625.66791324
CGCCGCT651.08885375E-425.38584724
AGAGTAC26050.025.01881811
CGATATC1001.3105273E-724.74962817
ACGGGGC1151.9123036E-823.91192416
TGGTATC14050.023.685932
ACGATAT701.801606E-423.57032616
GAATTCG701.801606E-423.57032628
CGGGGTC952.3171906E-623.15754717
TCGTCGC952.3171906E-623.15754710
TATCAAC52050.023.0909672
CAACGCA50600.022.7699495
TACGGTT852.572031E-522.64599830
ATCAACG52300.022.5030123
GACTCGT1103.2900243E-722.4989477
CAGAGTA29200.022.41404710
Produced by FastQC (version 0.11.5)