FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138822
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATTACATTCCATTCCATTCGGGTTGTTCCATTCCATTCCATTCCAT5040.3630548472144185No Hit
CCCTTACTCTCCATTACATTCCATTCCATTCGGGTTGTTCCATTCCATTC3970.2859777268732622No Hit
GAATAGAATGGAATGGATTGGAATCAACGCGAGTGCAGGGGAATGGAATG3930.28309633919695726No Hit
CCATTACATTCCATTCCATTCGGGTTGTTCCATTCCATTCCATTCCATTC3610.260045237786518No Hit
GAATGGAACAACCCGAATGGAATGGAATGTAATGGAGAGTAAGGGAGTTG3420.2463586463240697No Hit
GAATATAATCAATCCGAATGTAATGGAATGGAATGGAACGGAATGGAATT3310.23843483021423117No Hit
ATTCTATTCCATTCCATTCCCTTCCTTTCCGTTCCATTCCATTCCATTCC2840.2045785250176485No Hit
GTAATGGAGAGTAAGGGAGTTGAATATAATCAATCCGAATGTAATGGAAT2790.20097679042226738No Hit
GAGTAAGGGAGTTGAATATAATCAATCCGAATGTAATGGAATGGAATGGA2610.18801054587889526No Hit
GTTCCATTCCATTCCATTCCATTCCACTCCATTCCATTGCACTCGGGTTG2400.1728832605782945No Hit
GTCCATTGCAGGCGGATTCTGGCAGAACTATTGCAGAGTTCTCCAACCCA2290.164959444468456No Hit
GAATGGAACGGAATGGAATTGAATGGAATGGAATGGAATGGAATGGAATG2140.15415424068231262No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2130.15343389376323638No Hit
GAATGGAATGGAATGGAATGGAATGGAATGGAACGGAAAGGAAGGGAATG2060.1483914653297028No Hit
CCCTTACACTCCATTACATTCCATTCCATTTGGGTAGTTTCCATTCCATT2030.14623042457247412No Hit
ATTCCATTCCATTCCCTTCCTTTCCGTTCCATTCCATTCCATTCCATTCC2020.14551007765339788No Hit
GAATGGAATCAACCCGAGTGCAATGGAATGGAGTGGAATGGAATGGAATG1950.1404676492198643No Hit
GAATGGAATGGAACGGAAAGGAAGGGAATGGAATGGAATAGAATGGAATG1940.13974730230078805No Hit
CCCCTGCACTCGCGTTGATTCCAATCCATTCCATTCTATTCCATTCCATT1940.13974730230078805No Hit
GAATGGAATAGAATGGAATGGATTGGAATCAACGCGAGTGCAGGGGAATG1930.1390269553817118No Hit
GAATGGAACGGAACGGAATGGAATGGAATGGAATGGAATGGAATGGAACG1900.13686591462448314No Hit
ATTATATTCAACTCCCTTACTCTCCATTACATTCCATTCCATTCGGGTTG1870.13470487386725447No Hit
GTAAAAAGCTGGCTACCCGAGTGCCAGGGCTAGAATCTGGCCTAGAATCT1850.133264180029102No Hit
GAATGGAATGGAACAACCCGAATGGAATGGAATGTAATGGAGAGTAAGGG1840.1325438331100258No Hit
ATTCCATTCCGTTCCATTCCATTCCATTACATTCGGATTGATTATATTCA1830.13182348619094955No Hit
GTAAGGGAGTTGAATATAATCAATCCGAATGTAATGGAATGGAATGGAAC1820.13110313927187334No Hit
GAATGGAACGGAAAGGAAGGGAATGGAATGGAATAGAATGGAATGGATTG1760.126781057757416No Hit
CAATGGAATGGAGTGGAATGGAATGGAATGGAATGGAACAACCCGAATGG1710.12317932316203485No Hit
CCATTCGGGTTGTTCCATTCCATTCCATTCCATTCCACTCCATTCCATTG1710.12317932316203485No Hit
CCATTGCACTCGGGTTGATTCCATTCCATTCCATTCCATTCCATTCCATT1680.12101828240480615No Hit
CTCCATTACATTCCATTCCATTTGGGTAGTTTCCATTCCATTCCATTCCA1670.12029793548572992No Hit
GAATAAACCCGAGTGCAATGGAATGGAGTGGAATGGAATGGAATGGAATG1640.11813689472850125No Hit
TTCCAATGCATTCCATTCCATTCGATTCCATTCGATTGCACTCGGGTTGA1620.1166962008903488No Hit
GAGTGGAATGGAATGGAATGGAATGGAACAACCCGAATGGAATGGAATGT1610.11597585397127255No Hit
GAATGGAATGGAATGGAATGGAATGGAATGGAATCAACCCGAGTGCAATG1600.11525550705219635No Hit
CCATTACATTCCATTCCATTTGGGTAGTTTCCATTCCATTCCATTCCATT1600.11525550705219635No Hit
ATTCCATTCCGTTCCGTTCCATTCCATTCCATTTCATTCTATTCGGGTTA1600.11525550705219635No Hit
ATTCCAATCCATGCCATTCCACTCGTGTTGATTCCATTCTTTCCATTCCA1560.11237411937589142No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.1116537724568152No Hit
GGATTGGAATCAACGCGAGTGCAGGGGAATGGAATGGAATGGAATGCAAT1540.11093342553773898No Hit
GAACGGAATGGAATGGAATGGAATGGAATGGAATGGAACGGAAAGGAAGG1540.11093342553773898No Hit
GTTCCATTCCATTCCATTACATTCGGATTGATTATATTCAACTCCCTTAC1510.1087723847805103No Hit
ACTCCCTTACTCTCCATTACATTCCATTCCATTCGGGTTGTTCCATTCCA1500.10805203786143405No Hit
ATTCCATTCCATTCAATTGCACTCGGGTTGATTCCATTCCATTCCATTCC1480.10661134402328162No Hit
GTAATGGAGAGTAAGGGAGTTGAATAGAATGAATCCGAATGTAATGTAAT1450.10445030326605292No Hit
CCATTACATTCGGATTGATTATATTCAACTCCCTTACTCTCCATTACATT1440.10372995634697671No Hit
ATTCCACTCCATTCCATTGCACTCGGGTTGATTCCATTCCATTCCATTCC1440.10372995634697671No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1430.10300960942790048No Hit
GAATGGAATGGAATGGAATGGAATGGAACGGAAAGGAAGGGAATGGAATG1420.10228926250882425No Hit
GGAATGGAGTGGAATGGAATGGAATGGAATGGAACAACCCGAATGGAATG1420.10228926250882425No Hit
GAATGGAATTGAATGGAATGGAATGGAATGGAATGGAATGGAATCAACCC1410.10156891558974802No Hit
ATTCCATTGCACTCGGGTTGATTCCATTCCATTCCATTCCATTCCATTCC1410.10156891558974802No Hit
GAATGGAATTGAATGGAATGGAATGGAATGCAATGGAATGGAATCAACCC1410.10156891558974802No Hit
GGTTAATTCCATTCCATTCCATTCAATTGCACTCGGGTTGATTCCATTCC1390.10012822175159555No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATT354.8042895E-448.5876966
TGGTATC1050.048.5876962
GTATCAA5100.047.5159071
CGACTTT756.2955223E-945.3485153
TCGACTT756.2955223E-945.3485152
GTGGTAT1051.8189894E-1244.5387231
GACTTTT801.1137672E-842.5142334
GTGCGGG209.308343E-442.4989250-51
GGGATTC409.2922285E-442.498927
ATGTGTA655.513926E-639.2439042
GATGTGT559.499694E-538.6493031
TATCAAC6400.038.5285222
GGAAGTA450.001658220737.7768148
GGGAAGT450.001658220737.7768147
GTGTATA1501.8189894E-1234.0113871
TGTGTAT751.47095925E-534.0113873
CGTGGAA250.002785709533.99913456-57
GGTATCA2150.033.6159061
AACGCAG7500.032.8776746
CAACGCA7550.032.6599435
Produced by FastQC (version 0.11.5)