FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3173729
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61500.19377835977804028No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT50280.15842562487219294No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47730.15039091239359126No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38690.12190706894003867No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT35200.11091054088108972No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33080.104230701487115No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC45800.043.8012772
GTGGTAT45550.042.9321251
GTATCAA165300.042.2035181
AGCGTCA5150.042.0894473
TATCAAC199250.036.1561052
GGTATCA83050.034.5523071
ATCAACG220450.032.6019633
TCAACGC225850.031.8600984
CAACGCA226000.031.7835355
AACGCAG246150.029.285786
GCGTCAG7800.028.3347244
TCGACTT16850.027.746172
GCAGCGT8550.025.8553331
CGCAGAG275900.025.8181868
GTCGTAC850.001224990925.0071371
ACGCAGA286150.024.997347
GCAGAGT287350.024.8485779
CAGCGTC9050.023.9514522
CGACTTT20000.023.5886613
TATACGT5500.023.1829554
Produced by FastQC (version 0.11.5)