FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3714580
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57750.155468451345778No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT45910.123594053701899No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44990.12111732685794895No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT38850.043.330871
TGGTATC40100.041.865132
GTATCAA146950.039.9211241
TATCAAC169700.035.1879732
GGTATCA73700.033.9158821
ATCAACG181800.033.1732643
TCAACGC186350.032.317684
CAACGCA188000.031.9453335
GTCGACT18950.030.2843171
AACGCAG199700.030.1387926
CGACTTT19400.029.1373233
AGCGTCA7350.026.0210553
CGCAGAG235800.025.4879288
ACGCAGA236700.025.4265887
TCGACTT22300.025.3481662
GCAGAGT256450.023.4687259
CGTCAGA8400.023.275645
TCGAGGG54300.023.1697229
GTCGAGG105350.022.10938
AGTCGAG115550.021.6654877
Produced by FastQC (version 0.11.5)