FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3714580
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG201820.5433184909195656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT44150.037.6512151
TGGTATC45700.035.874062
GTATCAA176900.028.8128281
TATCAAC206700.024.4599462
AGAGTAC106650.024.1598811
ATCAACG219000.022.8802723
GGTATCA83500.022.5116331
TCAACGC222250.022.3703444
CAGAGTA116800.022.31934710
CAACGCA223800.022.2154125
AGTACGG116350.021.81480413
AACGCAG232200.021.6012386
GAGTACG120700.021.09695212
GTACGGG127300.019.6788714
CGGGAAA32750.019.48042917
ACGGGAA39150.019.3859716
TACGGGA50850.019.03539315
TACGGGG64550.018.9997915
GGACCGT1754.090907E-918.8564176
CGAGGGG19800.018.61039510
Produced by FastQC (version 0.11.5)