FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2178175
Sequences flagged as poor quality0
Sequence length61
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG122390.5618924099303315No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGA32680.15003385862017515No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGG23060.1058684449137466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC33700.040.816372
GTGGTAT34700.040.529871
GTATCAA146850.029.8555871
CGGTACC653.2090393E-629.61348217
TGCGACG852.7113856E-829.11777922
CGACGAG952.4974725E-928.947524
GTACTAG1303.6379788E-1227.522241
ATCGTCA1004.323738E-927.49823410
TCGATGC400.00787712627.49823228
AGAGTAC86700.026.35643811
TATCAAC168300.025.9573652
ACGGGTA3650.025.61479216
CAGAGTA89800.025.4465810
GGTATCA64650.025.2446821
GTCGTCA2550.024.80403524
AGGTCGT2600.024.32703422
CTAGTAC2800.023.5731623
ATCAACG187750.023.0855853
GAGTACG97700.023.05123112
CAACGCA189850.022.6387865
Produced by FastQC (version 0.11.5)