FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4026728
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG149010.3700523104614963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT44700.035.8884431
TGGTATC45150.034.7838552
GTATCAA166000.027.8812051
TATCAAC184650.024.770932
AGAGTAC98600.022.89760811
GGTATCA80100.022.5697351
ATCAACG201050.022.4730873
CAACGCA203200.022.0582775
TTAAACG5000.022.00010122
TCAACGC203800.021.9933364
TACGGGT5450.021.69615
AACGCAG214500.021.0757086
CAGAGTA111450.020.62766610
AGTACGG111100.020.02434513
GAGTACG116250.019.23186712
ACGCAGA249500.018.0096537
CGACTTT10850.017.995253
CGCAGAG250200.017.9372848
GTACGGG124850.017.5322514
GCAGAGT262050.017.1261569
Produced by FastQC (version 0.11.5)