FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24568
Sequences flagged as poor quality0
Sequence length91
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTGGGGG4471.8194399218495603No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGGGGGGG4261.7339628785411918No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTGGCGG3731.518235102572452No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCGATCAG2080.8466297622924128No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGGGGCGG1410.5739172907847607No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTGGGGGGGG1230.5006512536633019No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTTGGGG1060.4314555519374796No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGTTGGCGG1040.4233148811462064No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGGGGGGGGGG930.37854119179420387No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGGTGGGG750.305275154672745No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTTGCGG750.305275154672745No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGTTGGGGG740.30120481927710846No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTGGGGGGGGGG670.2727124715076522No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGTGTGGCGG630.25643112992510586No Hit
GTGTATAAGAGACAGATACACATCTCCGAGCCCACGAGACCGATCAGTAT620.2523607945294692No Hit
GAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCG610.2482904591338326No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGGGGGGGGGGGG610.2482904591338326No Hit
GTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCGATCAGTA600.24422012373819602No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGGGTGGCGG600.24422012373819602No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGTGTGGGGG530.2157277759687398No Hit
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGGGGGGGG510.20758710517746662No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGTGGGGGG500.20351676978183No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTGGCGGGGG480.19537609899055683No Hit
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTGGGGGG410.1668837512211006No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGTTTGGCGG410.1668837512211006No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGATGGCGG400.16281341582546402No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAA390.1587430804298274No Hit
GATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCGAT380.15467274503419082No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTGGCGGGGT350.142461738847281No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGGGGCGGGGG350.142461738847281No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTGGGGTGGCGG340.1383914034516444No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTGGGGTGGGGG340.1383914034516444No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTGGGGGGGT330.13432106805600783No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGTTTGGGG310.1261803972647346No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGGTGCGG310.1261803972647346No Hit
GGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACC310.1261803972647346No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA300.12211006186909801No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTGGCGGGGGGG300.12211006186909801No Hit
TATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTGGCGGGG290.11803972647346142No Hit
TATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTGGGGGGG280.11396939107782482No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGTTTGCGG260.1058287202865516No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTGGGCG260.1058287202865516No Hit
TATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGGGGGGGGG260.1058287202865516No Hit
GCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAG260.1058287202865516No Hit
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTGGCGGG260.1058287202865516No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGGGTGGGGG250.101758384890915No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCGT200.003186020863.751
AAACACA250.007706216551.01
CAGCGTC250.007706216551.02
CTTATAC10100.047.97031
TTATACA10800.045.6481482
TATACAC11000.044.818183
CAGAGTC450.001631543537.777784
AGAGTCG450.001631543537.777785
TACACAT14050.035.996445
ATACACA13900.035.773384
GAGTCGA500.002736415534.06
CCCACGC402.2628463E-531.87518-19
ACACATC16650.030.3753786
GGTTGCG353.8890526E-430.35714142-43
ACATCTC16850.030.0148378
CACATCT16900.029.9260377
CATCTCC16800.029.851199
GATGGAT600.00667856828.3333362
ACGCAGA600.00667856828.3333361
TAAAAAT300.006743263528.33333664-65
Produced by FastQC (version 0.11.5)