FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24568
Sequences flagged as poor quality0
Sequence length61
%GC76

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1109645.16444154998372No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG320.1302507326603712No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG290.11803972647346142No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG270.10989905568218822No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG270.10989905568218822No Hit
GGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG260.1058287202865516No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGG260.1058287202865516No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGCT202.563426E-455.020
TATAAGC202.563426E-455.019
GGCTCGG202.563426E-455.05
AAGCTGT202.563426E-455.022
GGGCTCG202.563426E-455.04
CGGAGAT257.706718E-444.09
TAAGCTG257.706718E-444.021
GTGTATA257.706718E-444.016
GATGTGT257.706718E-444.013
TCGGAGA257.706718E-444.08
CGTGGGC257.706718E-444.01
GCTCGGA257.706718E-444.06
AGATGTG257.706718E-444.012
CTCGGAG257.706718E-444.07
TGTATAA257.706718E-444.017
AGCTGTC257.706718E-444.023
TGTGTAT257.706718E-444.015
ATGTGTA300.00188919536.66666814
GTATAAG300.00188919536.66666818
GTGGGCT300.00188919536.6666682
Produced by FastQC (version 0.11.5)