FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21111
Sequences flagged as poor quality0
Sequence length91
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTGGGGG3961.8757993463123492No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGGGGGGG3561.686324664866657No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTGGCGG2921.3831651745535503No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCGATCAG1450.6868457202406328No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGGGGCGG1220.57789777840936No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA1000.47368670361422954No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTTGGGG990.4689498365780873No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGTTGGCGG870.41210743214437967No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGGTGGGG800.37894936289138365No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA790.37421249585524136No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGGGGGGGGGG790.37421249585524136No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAA780.36947562881909907No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTGGGGGGGG710.336317559566103No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAA690.3268438254938184No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTTGCGG660.3126332243853915No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGGGTGGCGG600.2842120221685377No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.2794751551323954No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGTTGGGGG560.26526455402396854No Hit
GTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCGATCAGTA510.24158021884325706No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGTGTGGCGG490.23210648477097248No Hit
CAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAA480.2273696177348302No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAA450.2131590166264033No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGATGGCGG450.2131590166264033No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.208422149590261No Hit
GTGTATAAGAGACAGATACACATCTCCGAGCCCACGAGACCGATCAGTAT440.208422149590261No Hit
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGGGGGGGG440.208422149590261No Hit
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTGGGGGG400.18947468144569182No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTGGCGGGGG390.18473781440954953No Hit
GCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAG370.17526408033726493No Hit
GAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCG370.17526408033726493No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.17526408033726493No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTGGCGGGGT360.17052721330112264No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGTGGGGGG350.16579034626498035No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGTGTGGGGG340.16105347922883806No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGGGGGGGGGGGG340.16105347922883806No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTGGGGGGGGGG330.15631661219269574No Hit
TATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTGGGGGGG330.15631661219269574No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTGGGGTGGCGG320.15157974515655345No Hit
GATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCGAT320.15157974515655345No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGTTTGGCGG320.15157974515655345No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGGTGCGG310.14684287812041116No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTGGGGTGGGGG300.14210601108426885No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGC300.14210601108426885No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTGGCGGGGGGG300.14210601108426885No Hit
ATGCCGGCCAGAACCTGTTGGATCAACAACCCCAATCCCTGTCTCTTATA300.14210601108426885No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCA290.13736914404812656No Hit
GGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.13736914404812656No Hit
TATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGGGGGGGGG280.13263227701198427No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAA270.12789540997584198No Hit
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTGGCGGG270.12789540997584198No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT240.1136848088674151No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTGCCGG240.1136848088674151No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGGGGGCG240.1136848088674151No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGTTTGGGG220.1042110747951305No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.1042110747951305No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTGGGGGGGT220.1042110747951305No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACTT258.94628E-568.000012
CGACTTT258.94628E-568.000013
TCGAGGG258.94628E-568.000019
GTATCAA700.066.785721
TATCAAC700.066.785722
GCCGCGA200.003179786563.755
GACTTTT302.2046894E-456.6666644
ATCAACG850.055.03
TCAACGC900.051.9444474
CTTATAC9950.051.6834141
GCGCCGC250.00769123151.0000043
CGAGATG250.00769123151.0000049
AGCGCCG250.00769123151.0000042
AGACAGG250.00769123151.0000048
CGCCGCG250.00769123151.0000044
GCGAGAT250.00769123151.0000048
CGCGAGA250.00769123151.0000047
CGTCAGA354.719336E-448.571432
GTCGACT354.719336E-448.571431
TTATACA10750.047.8372082
Produced by FastQC (version 0.11.5)