FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21111
Sequences flagged as poor quality0
Sequence length61
%GC74

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG927443.92970489318365No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAA520.24631708587939935No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAA280.13263227701198427No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAA270.12789540997584198No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATCTGTCTCTTATACACATCTGACG250.11842167590355739No Hit
GGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG250.11842167590355739No Hit
GGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG230.1089479418312728No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.1042110747951305No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAA220.1042110747951305No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA900.045.8333361
TATCAAC1100.037.52
ATCAACG1150.035.8695683
TCAACGC1200.032.0833324
CAACGCA1300.029.6153855
TCGAGGT752.7207352E-729.33333615
AACGCAG1400.027.56
CGAGGTT705.3671265E-627.516
AGGTTTT759.174404E-625.66666818
CGCTGCC651.0402139E-425.38461555
GAGGTTT801.51335025E-524.062517
ACGCTGC701.7204444E-423.57142854
AGAGTCG1750.023.57142811
ACGCAGA1703.6379788E-1222.6470587
CAGAGTC1703.6379788E-1222.64705810
GCAGAGT1703.6379788E-1222.6470589
CGCAGAG1703.6379788E-1222.6470588
GACGCTG804.2465763E-420.62553
GAGTCGA1904.5838533E-1018.8157912
GTCGAGG1502.8137583E-718.33333414
Produced by FastQC (version 0.11.5)