FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1566390
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG144700.9237801569213286No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA35850.22887020473828357No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAAC28560.18233007105510124No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAA27910.17818040207100402No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTT25600.16343311691213555No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGA23460.14977112979526172No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCA20580.13138490414264647No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCT17480.11159417514156757No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCA17450.11140265195768616No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGT16760.10699761872841375No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16400.10469934052183684No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAAT16250.10374172460242978No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAG16220.10355020141854837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT29600.041.645361
TGGTATC30900.039.25242
CGACAAC651.9790605E-938.07917832
CGCGTTC658.3973646E-833.84599723
GAACCGT1200.032.0811356
GTATCAA140150.030.4900861
TGCTTAA3750.030.06652619
ACAACGA952.4956535E-928.94908534
CTGCTTA4000.028.87394318
TGCGACA904.7206413E-827.5007530
GCTTAAC705.708869E-627.49987420
ATCGCGT1203.6379788E-1127.49987221
CGAGTGA507.035885E-427.49811610
AGCATCG4500.026.89048245
ACTGCTT4300.026.85948417
GCCGTCA4300.026.85862539
AGCCGTC4600.026.30338738
CAGAGTA83050.026.15714810
TATCAAC162350.026.1565912
TACCACT6350.025.98247313
Produced by FastQC (version 0.11.5)