FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1770715
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57300.32359809455502436No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT49590.2800563614133274No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42350.23916892328805028No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41490.23431212815162236No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT35980.20319475466125267No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34090.19252110023352148No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30570.17264212479139784No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA29450.1663169962416312No Hit
GTATCAACGCAGAGTACGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA25170.14214596928359446No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG25150.1420330205594915No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24780.13994346916358646No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA20610.1163936601881161No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT35050.061.48881
TGGTATC37100.058.7670252
GCGTCAG4500.046.2779164
GTATCAA160100.042.8536951
GCATCGT2250.041.562728
AGCGTCA5100.040.8334543
GGTATCA75400.040.422571
GTCAGAT5300.039.3003436
GTACCGA450.001661650437.7853621
TATCAAC195400.036.605792
GTACTAG2700.036.2109681
CGATACT500.002788578634.002025
ATCAACG219450.032.729653
TCAACGC225900.031.8515784
CAACGCA229150.031.4757985
CGACTTT16950.031.091543
TCGACTT17100.031.0673432
CGTCAGA6750.030.8536875
TACTAGG3350.030.4478532
AACGCAG246100.029.4847876
Produced by FastQC (version 0.11.5)