FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1770715
Sequences flagged as poor quality0
Sequence length61
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105010.5930372759026721No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21610.12204109639326487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT37450.045.063631
TGGTATC37850.044.7701642
TGCTACG651.9808795E-938.0753219
TTCGACC300.001926569236.6640939
ATCGTCA1750.036.14133510
ACGAGTC1600.036.09324626
GCGACGT401.9017993E-434.37549647
CGACGAG1700.033.9701224
ATGCGAC1800.033.61064521
GCGACGA1850.031.21578423
GTATCAA182200.030.9456121
GCATCGT2250.028.1099288
GTACTAG2900.027.5307161
TTTCGGA400.00787479727.49961921
TCTCGGA400.00787588227.49884212
CTTCGGG507.035165E-427.4988414
AGAGTAC123050.026.86193311
TATCAAC213250.026.6640012
GATACCA8550.026.3731611
CAATGCG2400.026.35305819
Produced by FastQC (version 0.11.5)