FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1230755
Sequences flagged as poor quality0
Sequence length91
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39320.3194786939723991No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA34300.2786907223614773No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30220.2455403390601704No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT30130.2448090806049945No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29420.23904026390305136No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT21640.17582703300006905No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20620.16753943717474234No Hit
CTGTAGTCCTTTCTCTCAATTTTCTTTTAAATACATTTTTTACTCCATGA20240.1644519014751108No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT19140.1555142981340722No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18210.14795796076392134No Hit
GTTTAAGTCACCTTCATTTTAATCTAAAAGCATTGCCCTTCTATTGGTAT18100.14706420042981747No Hit
CTTCTATCTTCTCCAAATTGTTTCATCCTACCACTCCCAATTAATCTTTC17490.14210789312251423No Hit
CAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATGCGTTAACTAGGC15730.12780772777685243No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGA15500.12593895616918072No Hit
GATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAA14900.12106389980134145No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14110.11464507558368643No Hit
GCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCG13980.11358881337065459No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTT13960.11342631149172662No Hit
TTCCCGTACTTCTGTCTTCCAGTTTTCCACTTCAAACTTCTATCTTCTCC13940.11326380961279865No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG13800.11212629646030281No Hit
AATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT12960.10530121754532787No Hit
TAGTAAATGCGTTAACTAGGCTTTAAATGACGCAATTCTCCCTGCGTCAT12630.10261993654301627No Hit
GACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACA12630.10261993654301627No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG12580.10221368184569633No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC26700.050.1441152
GTGGTAT27300.048.429131
GTATCAA109050.044.0905841
TATCAAC131550.037.6728442
GGTATCA51250.036.0831381
GTCCTAG858.695606E-735.002566
AGCGTCA3100.034.27673
CTACGGG500.00278528734.0093961
ATCCACG500.002788062734.0024872
TACCTAC250.002789712733.99972582-83
ATCAACG148400.033.5098653
TCAACGC150000.033.2090954
TCGACTT12700.033.1323432
CAACGCA153750.032.537345
CGACTTT13200.032.1993263
GTCTAGA1351.0149961E-931.4901811
TGCTACG759.276846E-1131.16641218-19
CGTATCA703.9370215E-430.3655321
GCGGTAT853.484464E-530.008291
GTACACG853.4893004E-530.0021956
Produced by FastQC (version 0.11.5)