FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25173
Sequences flagged as poor quality0
Sequence length91
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT3201.2712032733484289No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTGCAGCT1870.7428594128629882No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGG1370.5442339014022961No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGGGGGTGGCGG1290.5124538195685854No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGGGTGGCGG870.34560838994160414No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGGCGG840.3336908592539626No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGGGGGTGGGGG790.3138283081078934No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGGCGT790.3138283081078934No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGG680.2701306955865411No Hit
GTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAA650.2582131648988996No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGT640.25424065466968576No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGGGTGTGGCGG620.2462956342112581No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGGATGGCGG590.23437810352361657No Hit
GCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAG590.23437810352361657No Hit
GTGTATAAGAGACAGATACACATCTCCGAGCCCACGAGACTGCAGCTAAT560.22246057283597506No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGGGGTTGGCGG550.2184880626067612No Hit
GAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTG550.2184880626067612No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGGCGG530.21054304214833353No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTGTGGCGG530.21054304214833353No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGGGGGTTGCGG410.16287291939776743No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTTTGCCGT410.16287291939776743No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGGATGCCGG410.16287291939776743No Hit
GATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTGCA390.15492789893933978No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGGATGGCGG390.15492789893933978No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGGTTGGCGG380.15095538871012593No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGGGGGTTGGGG360.14301036825169824No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTGGGGTGGCGG350.13903785802248442No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGGATGCCGG340.13506534779327056No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTGTGGCGG330.13109283756405674No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC330.13109283756405674No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGGGGGGGGGGG320.12712032733484288No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA310.12314781710562905No Hit
GGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACT310.12314781710562905No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGGATGCCGT300.1191753068764152No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTTTGGCGT300.1191753068764152No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTGGTATGGCGG290.11520279664720137No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.11123028641798753No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTGGGATGGCGG280.11123028641798753No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTGGTGTGGCGG270.10725777618877369No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGGGTGGCGG270.10725777618877369No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGGGGGGGGCGG270.10725777618877369No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGGGTATGGCGG270.10725777618877369No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTGTGCCGG260.10328526595955985No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGGCGT260.10328526595955985No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAT150.001017811584.999991
GCGTCAG455.5333658E-966.1111151
TACAAAT200.003186930263.755
CTTATAC11650.057.639491
TTATACA12700.053.2086642
TATACAC13350.050.936333
CGTCAGA605.3592885E-849.5833322
GTATCAA409.146295E-442.51
ATACACA16550.041.0876124
TACACAT16800.040.476195
TCAACGC450.001632306337.777784
ATCAACG450.001632306337.777783
GAGATGG450.001632306337.777781
CAACGCA450.001632306337.777785
ACACATC18950.036.1081776
ACATCTC19400.035.4896938
CTAAGCG301.5739717E-435.41666434-35
TATTGCG301.5739717E-435.41666442-43
CGTGGGA301.5739717E-435.41666456-57
AGAGACA2400.035.4166648
Produced by FastQC (version 0.11.5)