FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25173
Sequences flagged as poor quality0
Sequence length61
%GC80

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1250049.656377865173No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG330.13109283756405674No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGG280.11123028641798753No Hit
GGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG280.11123028641798753No Hit
GGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG270.10725777618877369No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC501.7505898E-533.03
TATCAAC501.7505898E-533.01
AACGCAG553.3584263E-530.05
ATCCAAC553.3584263E-530.020
CAACAGG553.3584263E-530.023
GATCCAA553.3584263E-530.019
ATCAACG553.3584263E-530.02
CAACGCA553.3584263E-530.04
CCAACAG606.07939E-527.522
TTGTTGA651.04809464E-425.38461514
ATTGGGG651.04809464E-425.3846157
GGATTGG651.04809464E-425.3846155
GATTGGG651.04809464E-425.3846156
TTGGGGT651.04809464E-425.3846158
TTGATCC651.04809464E-425.38461517
AGGTTCT651.04809464E-425.38461527
CAGGTTC651.04809464E-425.38461526
AACAGGT651.04809464E-425.38461524
TCCAACA651.04809464E-425.38461521
ACAGGTT651.04809464E-425.38461525
Produced by FastQC (version 0.11.5)