FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179256
Sequences flagged as poor quality0
Sequence length91
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15840.8836524300441826No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12390.6911902530459232No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT9380.5232739768822243No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8250.4602356406480118No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT7280.4061230865354577No Hit
GGTTATACCTGCTTGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAAA4900.2733520774757888No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT4550.25382692908466103No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4380.244343285580399No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4300.2398803945195698No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3840.21421877091980182No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3360.18744142455482662No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAA3300.1840942562592047No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3280.18297853349399743No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3150.17572633552014996No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3090.17237916722452803No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT2680.14950685053777837No Hit
ATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2640.14727540500736377No Hit
GGGTTATACCTGCTTGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAA2500.13946534565091265No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2370.1322131476770652No Hit
ACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2370.1322131476770652No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT2200.12272950417280314No Hit
GTATCAACGCAGAGTACGGGCACGTATAAGACGAGCAAAAAAAAAAAAAA2070.11547730619895569No Hit
ATACCTGCTTGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAA1950.10878296960771187No Hit
GTATCAACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT1910.10655152407729727No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCATT150.001028159784.999456
ATCCACG150.001028159784.999452
TCATTGC200.003219128463.749588
TCCACGC200.003219128463.749583
CTCTGCA401.45333215E-553.124659
TTATCAA509.06306E-751.028131
AGAACTT250.00778578850.9996645
CTAGGCA354.8161767E-448.571114
TCGACTT4250.047.9996872
GAATCAA452.90725E-547.2482721
TCTCTGC551.7548573E-646.3633358
GTCAGAT551.7548573E-646.3633352
CAGATGT551.7548573E-646.3633354
CGACTTT4600.044.3475343
CACATGT603.1938016E-642.4997258
CGTGGTC209.3111175E-442.4997248-49
TATTCTC505.433718E-542.499725
TCTCGAA409.298636E-442.499727
ATTCTCT505.433718E-542.499726
TGGTATC9400.042.0475922
Produced by FastQC (version 0.11.5)