FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179256
Sequences flagged as poor quality0
Sequence length61
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG94875.292430936760834No Hit
GGTTATACCTGCTTGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2790.15564332574641854No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2510.1400232070335163No Hit
GTATCAACGCAGAGTACGGGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAA1840.10264649439907171No Hit
GGGTTATACCTGCTTGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1840.10264649439907171No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTCT150.00577359155.00502432
GTCGAAT150.0057799754.9896814
GACACCT150.0057799754.9896839
CCCGTAG150.0057799754.989689
GGGACGA150.0057799754.9896818
GTTATCA300.0019125536.7007371
GTGGTAT8800.036.5964741
TGGTATC9200.034.696442
GCACCTA401.897259E-434.36854639
TCGACTT2700.033.632952
CGGGACG501.8138086E-532.99380517
CGACTTT2700.032.595573
ACAGCAC350.00408866231.43144236
GACTTTT2850.030.8713994
ACCTACA453.787059E-430.54982441
GGATAGC653.1835843E-629.61808820
GTATCAA39450.028.9559021
CGGGGAC952.4701876E-928.94193817
GTCGACT3350.028.7580381
CGGGGTA2500.028.59463317
Produced by FastQC (version 0.11.5)