FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416330
Sequences flagged as poor quality0
Sequence length91
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18510.44459923618283576No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15640.3756635361371989No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15020.36077150337472674No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT13950.33507073715562175No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10630.2553263036533519No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT10600.2546057214229097No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9790.23515000120097038No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8350.2005620541397449No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA7410.17798381091922272No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG7010.1683760478466601No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC6860.16477313669444912No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA6670.16020944923498184No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC5820.1397929527057863No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC5680.13643023563038933No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC5490.1318665481709221No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAA5400.1297048014795955No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG5280.12682247255782672No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT5160.12394014363605795No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4680.11241082794898277No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4620.11096966348809838No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4610.11072946941128431No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA4610.11072946941128431No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA4600.11048927533447025No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT4460.10712655825907333No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATTTT302.6417329E-670.848955
CTATCAA751.8189894E-1256.679161
TCGACTT5950.052.148092
TGGTATC9600.051.3593182
CAGTATC250.007785990451.0112461
GTGGTAT9600.050.9226841
CGACTTT6350.048.863173
TCAATGG452.9217259E-547.226963
TCGACAT452.9217259E-547.226962
GTATCAA52400.046.1599851
ATTCTCT1200.046.051826
TTCTCTG1200.046.0462887
GACTTTT6950.044.6447684
TATTCTC1350.044.0837945
ATGGCAC505.4388784E-542.5093736
GTACTAG801.12413545E-842.5093731
GAAGTCC801.12522685E-842.5042658
CAATGGC505.4427353E-542.5042654
CTTAACG401.1288648E-842.49915720-21
TAGGCAT951.1386874E-940.2720385
Produced by FastQC (version 0.11.5)