FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804764
Sequences flagged as poor quality0
Sequence length91
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16350.2031651515226824No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12080.15010611806691154No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT9220.11456774905438116No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8750.108727527573301No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGCC302.2537597E-456.6720967
CGTACCG409.3133515E-442.504079
TATACGT1550.041.132974
GTATCAA39400.040.9011461
ATACGTG1300.039.2345285
GGTATCA19850.037.2718731
AGCGTCA2500.035.703423
TATCAAC46950.035.2163662
CGACTTT3350.034.257013
GCGGTAT500.002782091534.0159381
ACCGGTC250.002788431734.002212-13
TCGCGTA250.00278970633.9990382-83
TCGACTT3450.033.2640532
ATCAACG51350.032.447123
CAACGCA51650.032.094075
GCGTCAG2650.032.0785454
TCAACGC53250.031.0499234
GTCGACA550.004437037730.9235781
GTGGTAT11050.030.398861
AACGCAG55600.029.8923116
Produced by FastQC (version 0.11.5)