FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922005
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10170.1103030894626385No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA1200.042.5019263
GGTATCA8850.039.8627241
GTATCAA18650.038.971711
GTAGACG450.001662505837.7794884
GACCGTC250.002789543733.99969522-23
TATACGG1404.1836756E-1133.394372
ATCAACG22300.032.5911643
GTATACG802.3051372E-531.8781741
TATCAAC22700.031.8296342
CAACGCA23150.031.394515
TCAACGC23350.030.9435864
GTCGACT1956.91216E-1126.1564481
CGCAGAG28050.025.9102658
GTGGTAT5650.025.5777651
AACGCAG28750.025.576466
ACGCAGA30450.023.8680767
TGGTATC5600.023.5278512
CGACTTT1852.9202056E-822.9740143
GTGCTAC1353.1703872E-522.039231
GCAGAGT33600.021.6304449
Produced by FastQC (version 0.11.5)