FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922005
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20760.22516146875559245No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGG257.821205E-444.03931
GTGGTAT6100.034.2928961
TGGTATC6100.033.3687172
CCGCGAC350.00409981131.4276453
GTATCAA24550.029.374481
GCGACGC400.007873476527.49918737
ACGGCGA400.007873476527.49918734
TATCAAC29250.024.544392
AGAGTAC13700.024.48832711
GGTATCA11250.024.2216131
ATCAACG29500.024.051443
CAACGCA29650.023.5574825
ACGGTGC600.002035576622.91598936
TCAACGC30800.022.588624
GACGTTG752.872636E-421.9993551
CGGGATT1254.622416E-821.9993517
GTACGCT752.872636E-421.9993536
AACGCAG33300.021.5534156
ACCGTGC650.00323982621.1532218
CAGAGTA16050.020.56013910
Produced by FastQC (version 0.11.5)