FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1131670
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16830.148718265925579No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12330.10895402369948837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17250.039.178761
GTATCAA33650.035.7473681
AGCGTCA2850.034.2979433
GCGTCAG3200.031.8761274
TATCAAC38800.031.5461122
GTGGTAT8750.031.0895581
TGGTATC8850.030.734192
ATCAACG40400.030.0863693
TCAACGC40800.029.4802074
CAACGCA41550.029.051655
AACGCAG46900.025.9189076
CGTCAGA3950.025.8248395
CGACTTT4150.025.6021843
CGCAGAG49500.024.299918
GCATCGT2451.8189894E-1224.2876438
TACGGGT2152.3283064E-1023.721774
GTACTAG3100.023.3093361
ACGCAGA53400.022.6843877
GCAGAGT54550.022.2840829
GGCATCG2305.4205884E-1022.1756787
Produced by FastQC (version 0.11.5)