FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1131670
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17990.15896860392163795No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCGA257.856989E-443.99936348
CGGCGAT257.8586984E-443.9974249
GTGGTAT9200.035.8912471
TGGTATC9100.035.6730082
GTATCAA41800.029.688991
GGTATCA17550.026.34071
AGAGTAC24800.025.393911
TATCAAC49000.025.1524962
CAGAGTA27200.023.86100210
TGCGACG701.7996975E-423.5731722
ATCAACG52350.023.2698843
TCAACGC51900.023.2597144
CGCGTAT600.00203463622.9183624
TCGCGTA600.00203463622.9183623
CAACGCA53300.022.6487665
TCGTCTA852.570564E-522.6467354
CGACGAG752.8711854E-422.00162724
AACGCAG56000.021.8022946
GAGTACG29150.021.32138812
TGTGTCG650.003240821921.1526076
Produced by FastQC (version 0.11.5)