FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2998673
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG146540.48868282737064034No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGA30800.10271209965207943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT26600.033.3164631
GTATCAA108150.028.985571
TGGTATC29200.028.9225982
TATCAAC122450.025.274092
AGAGTAC66000.024.49860211
ATCAACG130550.023.4043083
CAGAGTA70250.023.05562410
TCAACGC133800.022.7524644
CAACGCA134100.022.680685
GGTATCA50150.022.665421
AACGCAG143600.021.486626
GAGTACG74200.021.42061212
CTAACCG904.000143E-521.387679
AGTACGG77900.020.72089813
TACGGGG39850.020.35643215
CGGGGTA5450.020.18233517
CGAGGGG12900.019.18495210
CGCAGAG157100.019.1316268
TAGGTCG1452.217057E-718.96538521
CGACTTT6950.018.5982323
Produced by FastQC (version 0.11.5)