FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284559
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62072.1812699651038976No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3000.10542629120850158No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGGT150.00577974154.99859617
TTCAGCG251.1854245E-554.99859254
CGATTCA251.1854245E-554.99859251
GATTCAG251.1854245E-554.99859252
CGACGCT303.485075E-545.8321628
ATTCAGC303.485075E-545.8321653
ACGCAGT257.847475E-443.99887527
AGAACGG257.847475E-443.99887513
AGACACG257.847475E-443.99887523
GCCTTTA403.9300958E-641.2489538
GCCTTAG458.8223605E-636.6657332
CCTAAAG300.001923433536.6657332
GTGTCAC300.001923433536.6657334
ACTCGGA458.8223605E-636.6657311
AAGTGTC300.001923433536.6657332
GCCAATC300.001923433536.6657321
TCGACTT2000.035.7616542
GGCCTTA558.6711043E-734.99910431
CGCTGCA401.8982468E-434.37412331
CTTAAAC501.8155277E-532.99915721
Produced by FastQC (version 0.11.5)