FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122
Sequences flagged as poor quality0
Sequence length91
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG10.819672131147541No Hit
CCATAAAAGCAACATCATAGAAACCAGATGATCACGATATCATATCGTGA10.819672131147541No Hit
AACATCCTGATTCAAACTATATAAACACCCTTCAAAGCCAAAGAACTCCC10.819672131147541No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTATTTAAAAAACAA10.819672131147541No Hit
TATACACATCTCCGCGCCCACGAGACACTGAGCGAGGGGGGGGGGGGGGT10.819672131147541No Hit
TCTGAACTCCAGTCACCGTGATATCTCGTATGCCGTCATATGGGTGTAAA10.819672131147541TruSeq Adapter, Index 25 (95% over 21bp)
GGGCTGACGCCGCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGCAG10.819672131147541No Hit
ACACATCTCCGAGCCCAAGAGACATGCGCAGATGGCGTGATACGGATTGT10.819672131147541No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGGGGGTGGCGG10.819672131147541No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGGGGGGGCGGGGG10.819672131147541No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGCTACGGCTCGTGTCGCGG10.819672131147541No Hit
GAGTACGGGGATAACATGTTGTTCTGTTTTGTAAAGTGTGTGTGCTTGGG10.819672131147541No Hit
CTTGATACGTATAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGACACT10.819672131147541No Hit
ACAGGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAA10.819672131147541No Hit
AATCCTTCAGGTTATCTTGAACTGCCTGACTTAGTAAAGACATAAAGAAG10.819672131147541No Hit
CGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAATAAAAACTGGGT10.819672131147541No Hit
CGATAGTAAGGTGAAACAAATAAATATCATTATAAAACATGGTGCAGGTT10.819672131147541No Hit
CTAATACACATCTACGAGCCAACTAGAAAATCATAGATGTCGTAATAGGT10.819672131147541No Hit
CTGCGTTTCAATCCTGGTGGTATACAGTCTCTGCGGGTCCAGACCAAACC10.819672131147541No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGGGGGGTGGGCG10.819672131147541No Hit
ATTCGATTCCATTCCGTTCCATTCCGTTCCATTCCATTCCATTCCATTCT10.819672131147541No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTGGGATTGCGG10.819672131147541No Hit
CAAAATAATCAAAAGAAATAAGTCATAAAAAATAGAGAGACAAAAGAATT10.819672131147541No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACCACGGGGGGGG10.819672131147541No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGGCCCA10.819672131147541No Hit
TCTATATCCAAGTTACCTCCACCTTCTAGCTCAGCTTCCAAACTGAGGAC10.819672131147541No Hit
GTATCAACGCAGAGTAGAGGGTTTATTTTAATATTTATGTTTTTTTATTG10.819672131147541No Hit
TATACACATCTACGAGCCCACGAGACTAGGCATGAGCTCGTATGCCGTCT10.819672131147541No Hit
GCATTCAGCTCACCGCTTGAGGAAGTAAAAGAAGATGATTTAATTCTGCA10.819672131147541No Hit
AGACAGTATCAACGCAGAGTACGGTGAAAAAAAAAAAAAAAAAAAAAAAA10.819672131147541No Hit
TTATACACATCTCCGAGCCCACGAGACAGGAAGAAATGTCTTATTGGGGG10.819672131147541No Hit
CATGGTCAGACAACAGCAAATGGGCACATAAATCCTATTATTTTAATTCC10.819672131147541No Hit
GTGTACACCCATATGAGCAATGATTGCACAAACTGCGATCACCTTATACA10.819672131147541No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTGGGGGGGCGG10.819672131147541No Hit
CTTATACACATCTCCGAGCCCACGCGACTAGCGCAACTCTCGGAATCCCG10.819672131147541No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAATTAG10.819672131147541No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGGGTTGACGTGGT10.819672131147541No Hit
CTTATACACATCTCCGAGCCCACGAGCCCCTAAGACATGGGGGGTGGCGG10.819672131147541No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAGGG10.819672131147541No Hit
CCGCTACATATATACGAAAAGGGAAGCTAGAATAGCTCAAGGAACTCGTG10.819672131147541No Hit
CTTATAAACCTCTCCGAGCCCACGAGACTAGGCATGTTCTAGTATGCCTT10.819672131147541No Hit
ACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTGGGATAT10.819672131147541No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.819672131147541No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG10.819672131147541No Hit
TTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG10.819672131147541No Hit
ATACACAACTCCGAGCCCACGAGACAAGAGGCAATCTCGTATTCCGTCTT10.819672131147541RNA PCR Primer, Index 48 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGCCCCTAAGCCCTCGGGTTGGGCGG10.819672131147541No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTATGGCGGGGT10.819672131147541No Hit
CAATTTAATTATGTACTTTTTCTTTTAAGAAAATCATATAACATTCCTTT10.819672131147541No Hit
GATAAAGTCCGAGAAATGTTTATGAAGACTGCCCATGTCACAGACCCCAG10.819672131147541No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATATCGTTTGGGCGCTG10.819672131147541No Hit
ATCAAAGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAC10.819672131147541No Hit
GTTTCCTCCTATGGGTTTCAAAACAATCACCATCATGCTATTAATGATAT10.819672131147541No Hit
TTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAGTG10.819672131147541No Hit
GTATAAGAGACAGTTATACACATCTCCGAGCCCACGAGACTAGGCATGAT10.819672131147541No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGGGGGATGGCGG10.819672131147541No Hit
TATCAACGCAGAGTACGGGCCGCTCGAGTTGTATATAATGTAAATATGGT10.819672131147541No Hit
GTTCTACACTGAATTCCACCCCACTGAAAAAGATGAGTATGCCACCCGGG10.819672131147541No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.819672131147541No Hit
TATCAACGCAGAGTCGAGCTTTTTTTTTTTATTTTATTTTTTTTTTATGT10.819672131147541No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGCGGGCGGGGGGCGGGGG10.819672131147541No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAATATCCTCCAGGGGATATAAAGACT10.819672131147541No Hit
GTCCTGACCCACTCGCCCAAGTAAGTGCACACGATTCAGGGATCTTTCAA10.819672131147541No Hit
AATTTATAGATTCAAATATAAAAAAAAAAAGATACCAGTTAATCAGTTCG10.819672131147541No Hit
CTTATACACATCTCCGAGCCCACGAGAACCTAAGACATGGGGTAAGGCGG10.819672131147541No Hit
CTTATACACATCTCCGCGCCCACGAGACTAAGCTTCTTGTGTTATGGCGG10.819672131147541No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT10.819672131147541TruSeq Adapter, Index 11 (95% over 21bp)
GTATCAACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTGG10.819672131147541No Hit
TCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGC10.819672131147541No Hit
TTATACACATCTCCGAGCCCACGAGCCAGGCAGAAATCGCGGGTTGCGTG10.819672131147541No Hit
CCGCAGCACACCATAGCAGGCCCTCCGCACAGCAACTGTCTCTTATACAC10.819672131147541No Hit
CTTATACACATCTCCGAGCCCACGATACGCTCATGAATGGAGGAATGCGG10.819672131147541No Hit
GCTCAGTACCTGGTACTAAGCACATTTGATCAATACTTGCTGAATAAAGA10.819672131147541No Hit
TATACACATCTCCGAGCCCACGAGACCAATCAGAAGATGAGATTGAGCTG10.819672131147541No Hit
TATCACATCTCCGAGCCCACGAGACTAGCGCTCATGCCGCGTGTGGGGGG10.819672131147541No Hit
CAAGGAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGAGCGCCTGG10.819672131147541No Hit
AAAAAAAAAAAACCCTTAAAAACAAAATCAAATAAAATCAGTTCTCTTAA10.819672131147541No Hit
GACTTAAACCGCTTAAAGTTTAGTTTAAAAGTTGTAGTTTATTAATATAA10.819672131147541No Hit
AATCTCCGACCCCACGCGACGCTCATTAAAGGCCCAATGGACAGGCTGCT10.819672131147541No Hit
GAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGCAGCTGTCTGTGAT10.819672131147541No Hit
CTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGGGTTTGCCGTC10.819672131147541No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGTAGTTGTGTTTGTTG10.819672131147541No Hit
GCAGAGTACGGGCAATTAGTAGCGCTCCGCTCAAGAGCTTACCCGCCTTA10.819672131147541No Hit
ATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCACCTATCACACA10.819672131147541No Hit
ACAGATACACATCTCCGAGACCACGAGCCTAGGCATGAGATCGTCTGCCG10.819672131147541No Hit
ACACAGCTCCGAGCCCACGAGACATGCGCAGATCGCGTATGCCGTGTTCG10.819672131147541No Hit
CAAACGTGCCATTGATAAATGCTGGAAGATTAGACACTTCCCCAACGAAT10.819672131147541No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAACAAAAAGG10.819672131147541No Hit
CTTATACACATCTCCGAGCCCACGCGACTAGGCATGATGTCGTATGCCGT10.819672131147541No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATGGCGTAGGCCGT10.819672131147541No Hit
TATATACATCTCCGAGCCCACGAAAATAGCGAACAGTGCCTATGCCGGGG10.819672131147541No Hit
CGTTTTACCAGTCCCTAGCAGTCGCGTCGCTCCCGCGGAGCGCATCTTTA10.819672131147541No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACCTGTCGGATGCCGT10.819672131147541No Hit
ATACACATCTCAGAGCACACTAGAAAGTCAGAAATTGGGCATTAGCGGAG10.819672131147541No Hit
CTTATACACATCTCCGAGCCCCCGAGACAGGCAGCAAGCGGGTCTGGGGG10.819672131147541No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.819672131147541No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.819672131147541No Hit
ATACACATCTCCGAGCCAACGAGACCCTAAGACATCGCCTTAGCCGTGGG10.819672131147541No Hit
AAATACACATCTCCGAGCCAACGAGACTAGAGCTCATGTGGTGTTACGTA10.819672131147541No Hit
ATATAAAAGCAGCATAAAGGTAAAAACCCAAAAAAAAAAAAAAAAATCTC10.819672131147541No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATGTGGTATGGCGGGTTGG10.819672131147541No Hit
GCGTTAACTAGGCTTTAAATGACGAAATTCTCCCTGCGTCATGGATTTCA10.819672131147541No Hit
GCGGTATCAACGCAGAGTACGGGATGGGTGTAATATTAAAAAGTGTTTTA10.819672131147541No Hit
CTTATACACATCTCCGAGCCCACCAGACTAGCGCGCATCTAGGATTCCGG10.819672131147541No Hit
GTATCAACGCAGAGTACGGGGGGAAAAAAAAAACTGTCTCTTATACACAT10.819672131147541No Hit
CATTAATAGCATGATGGTGATTGTTTTGAAAACCATAGGATGAAACCTCC10.819672131147541No Hit
CGTCTGAACTCCAGTCACCGTGATATCTCCTGAGCCTAACTCGGGTTGTA10.819672131147541TruSeq Adapter, Index 12 (95% over 22bp)
GTACGGAGAGAAAAACACAAGCAAATAACAAAACTACAAAACGAAGAATC10.819672131147541No Hit
GTATCAACGCAGAGTACGGGCAAAAAAAAAAACAAACAAAAACTAATATA10.819672131147541No Hit
TTATTAGACGAGCAAAAAAAAACAAAAAAAAATATAAAAAAAAATAGCGC10.819672131147541No Hit
GTATCAACGCAGAGTCCGGGGGGAAAATAAAATAAAAAAATATAAATAGG10.819672131147541No Hit
GGGCTCAACGAAGAGCCGAGTGTTGTTTTTTTTTTTTTAGTTTTTTATTG10.819672131147541No Hit
TTAAAAACATCTCCAATACAAAGATAATATTCATGATATATTATGCACAG10.819672131147541No Hit
GACTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATGTTTTTT10.819672131147541No Hit
CTTAGACACATCTCCGAGCCCACGAGACTAGCGCGCATGGGGTAGGCCGG10.819672131147541No Hit
GAGTACGGGGTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.819672131147541No Hit
CTTATACACATCTCCGAGCCCACTAGCCTAGCGCTCGAGGGCTGGCTGGG10.819672131147541No Hit
TTGTGGTATCAACGCAAAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT10.819672131147541No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGGGTTCCGGGGT10.819672131147541No Hit
ATACACATCTCCGAGCCCACGAGACCGGACTAGAGCTCGTATGCCGGCTG10.819672131147541No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTGGTTTTCCGTGGT10.819672131147541No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGGCGTATTGCG10.819672131147541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG1400.06.071428374-75
Produced by FastQC (version 0.11.5)