EGAF00005411706 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005411706
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	38463
Sequences flagged as poor quality	0
Sequence length	91
%GC	41

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA	515	1.3389491199334425	No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	408	1.060759691131737	No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	382	0.9931622598341263	No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA	344	0.8943660140914645	No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA	290	0.7539713490887346	No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT	275	0.7149728310324207	No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	202	0.52518004315836	No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	188	0.4887814263058004	No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT	175	0.454982710656995	No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC	163	0.42378389621194396	No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA	154	0.40038478537815564	No Hit
CCCTTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGA	134	0.34838676130307045	No Hit
GCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTA	122	0.3171879468580194	No Hit
CATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	116	0.30158853963549387	No Hit
GTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTC	112	0.29118893482047686	No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT	111	0.28858903361672256	No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATC	110	0.2859891324129683	No Hit
ATTGATAACCTTGTTTTGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA	108	0.2807893300054598	No Hit
CTTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATA	107	0.27818942880170555	No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAA	107	0.27818942880170555	No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	106	0.27558952759795124	No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCCTGTCTCTTA	101	0.26259002157918	No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	93	0.24179081194914592	No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA	91	0.23659100954163742	No Hit
ATGTACTGTCAAGTAGGAAAGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA	91	0.23659100954163742	No Hit
ATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTG	87	0.2261914047266204	No Hit
TTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATAC	87	0.2261914047266204	No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	83	0.21579179991160335	No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	83	0.21579179991160335	No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	82	0.2131918987078491	No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACCTGTC	81	0.21059199750409485	No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCTG	66	0.17159347944778097	No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	66	0.17159347944778097	No Hit
TATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAG	65	0.16899357824402675	No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	64	0.16639367704027247	No Hit
TATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAA	61	0.1585939734290097	No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACAT	60	0.15599407222525544	No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	60	0.15599407222525544	No Hit
ACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59	0.15339417102150119	No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAG	57	0.14819436861399266	No Hit
GATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCT	56	0.14559446741023843	No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGCTGTCTCT	56	0.14559446741023843	No Hit
TATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATCT	56	0.14559446741023843	No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54	0.1403946650027299	No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCCTGTCTCTTATACACA	54	0.1403946650027299	No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCCTGTCTCTT	53	0.13779476379897562	No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	53	0.13779476379897562	No Hit
GCCTTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGA	52	0.13519486259522137	No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52	0.13519486259522137	No Hit
ATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAA	51	0.13259496139146712	No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT	50	0.12999506018771287	No Hit
TTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	50	0.12999506018771287	No Hit
ATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGG	49	0.12739515898395862	No Hit
CATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	48	0.12479525778020435	No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCCT	48	0.12479525778020435	No Hit
CTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCT	48	0.12479525778020435	No Hit
GCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCCTGTCTCTT	48	0.12479525778020435	No Hit
GATATACGCATTGTGGCAGTACATCAAGGGCTGTCTCTTATACACATCTC	47	0.1221953565764501	No Hit
GTATCAACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46	0.11959545537269584	No Hit
GAATAAGCTGGTATCCCGTACTCTGCGTTGATACCTGTCTCTTATACACA	46	0.11959545537269584	No Hit
GATATACGCATTGTGGCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	45	0.11699555416894158	No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	44	0.11439565296518732	No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACAT	44	0.11439565296518732	No Hit
GTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACTGTCT	43	0.11179575176143307	No Hit
GTACTGTCAAGTAGGAAAGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	43	0.11179575176143307	No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT	43	0.11179575176143307	No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	42	0.1091958505576788	No Hit
ATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATCTC	42	0.1091958505576788	No Hit
TACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAA	42	0.1091958505576788	No Hit
AAACAAGGTTATCAATAATTGCCCCGTACTCTGCGTTGATACCTGTCTCT	41	0.10659594935392455	No Hit
ATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41	0.10659594935392455	No Hit
GTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCCTGT	39	0.10139614694641604	No Hit
GTGGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACAT	39	0.10139614694641604	No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATCTGTCTCTTATACACATCTC	39	0.10139614694641604	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCTTGA	20	2.9913963E-5	85.00001	1
AATAAGC	35	7.5659045E-8	72.85714	2
ATAAGCT	45	5.638867E-9	66.111115	3
AAGCTGG	45	5.638867E-9	66.111115	5
TAAGCTG	50	1.29639375E-8	59.499996	4
CTGGTAT	50	1.29639375E-8	59.499996	8
GAATAAG	50	1.29639375E-8	59.499996	1
AGCTGGT	50	1.29639375E-8	59.499996	6
CCTTGAT	75	1.8189894E-12	56.66667	2
GTGGTAT	130	0.0	55.57692	1
GCTGGTA	55	2.7504939E-8	54.090904	7
CCCTTGA	50	8.9103924E-7	50.999996	1
GGTATCA	350	0.0	46.142857	1
GTATCAA	815	0.0	45.368095	1
TGGTATC	180	0.0	42.500004	2
GAGACAG	40	9.2072645E-4	42.500004	7
CTTGATG	100	3.8198777E-11	42.499996	3
TATCAAC	905	0.0	40.38674	2
TAAGAGA	45	0.001643133	37.77778	4
AGAGACA	45	0.001643133	37.77778	6

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content