FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38463
Sequences flagged as poor quality0
Sequence length61
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6851.7809323245716664No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1960.5095806359358345No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATCTGACGCTGCCG1490.3873852793593843No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1050.272989626394197No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCCTGTCTCTTATACACATCTGA1050.272989626394197No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACCTGTCTCTTATACACA930.24179081194914592No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCG850.22099160231911189No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCTGTCTCTTATACA770.20019239268907782No Hit
GCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGA730.1897927878740608No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA710.18459298546655226No Hit
GTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACA600.15599407222525544No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGGGCTGTCTCT590.15339417102150119No Hit
GAATAAGCTGGTATCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGC520.13519486259522137No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGCTGTCTCTTA510.13259496139146712No Hit
CTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT480.12479525778020435No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCCTGTCTCTTATACACATCTG480.12479525778020435No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCCTGTCTCTTATAC480.12479525778020435No Hit
ATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACA470.1221953565764501No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATCTGACGCTGCC460.11959545537269584No Hit
CTTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTA460.11959545537269584No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAA420.1091958505576788No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGCTGTCTCTTATACACATCT420.1091958505576788No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACCTGTCTCTTATACAC400.1039960481501703No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTGACGCTGCCGA400.1039960481501703No Hit
CCCTTGATGTACTGCCACAATGCGTATATCCTGTCTCTTATACACATCTGACGCTGCCGAC400.1039960481501703No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGCA150.00574134255.02
CAGACGC202.5818942E-455.01
CAGTGGT150.00574134255.01
GTGGTAT2200.047.51
ATAATTG303.428242E-545.83333215
TAATTGC303.428242E-545.83333216
CGGGGCA257.761814E-444.017
CAATAAT257.761814E-444.013
ACGGGGC257.761814E-444.016
TATCAAT257.761814E-444.010
ATCAATA257.761814E-444.011
ATTGCCC358.512362E-539.28571318
AAGGTTA358.512362E-539.2857135
AGGTTAT358.512362E-539.2857136
CAAGGTT358.512362E-539.2857134
ACAAGGT358.512362E-539.2857133
TGGTATC2700.037.685192
ACGCCTG300.001902597436.66666829
TACGCCT300.001902597436.66666828
ATACGCC300.001902597436.66666827
Produced by FastQC (version 0.11.5)