FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148862
Sequences flagged as poor quality0
Sequence length91
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA10270.6899007134124222No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8650.5810750896803751No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT7130.4789670970428988No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6740.4527683357740726No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4640.3116980827880856No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA4630.31102631967862854No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT4620.31035455656917144No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA4210.28281226908143114No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAA3260.2189947736830084No Hit
GAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA2950.1981701172898389No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA2530.16995606669264152No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT2480.16659725114535612No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2450.16458196181698487No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2270.1524902258467574No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA2210.14845964719001492No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.12696322768738832No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1890.12696322768738832No Hit
ATTGATAACCTTGTTTTGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA1880.12629146457793122No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1710.11487149171716085No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1660.11151267616987545No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT1620.10882562373204713No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.10681033440367589No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3150.056.6651041
TGGTATC3800.045.8539962
GTATCAA18100.045.316421
GGTATCA8150.044.8453871
CAGGTAT906.657501E-1042.4988251
GTGCAAA701.8846185E-742.4988251
CATATAC505.4299326E-542.4988251
TATCAAC20650.039.9262582
ATCAACG23450.035.883873
AGGTATC955.0873496E-835.7884862
CAACGCA23800.035.1775975
TCAACGC24000.034.884454
CGACTTT1352.7284841E-1134.628673
TCGACTT1352.7284841E-1134.628672
GACGGTT500.002777028834.0104836
CTGCTAA500.002781609533.9990581
AACGCAG24800.033.9419146
GTCGACT1404.0017767E-1133.3919331
AGACGCA652.5431602E-432.6914062
GCAAAAC802.2942586E-531.8741193
Produced by FastQC (version 0.11.5)