FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148862
Sequences flagged as poor quality0
Sequence length61
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11750.7893216536120703No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3340.22436887855866505No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA2630.1766736977872123No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1860.12494793835901709No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAT150.005758838355.035971
TTTGATC150.00577417954.99899319
GTGGTAT6300.044.552931
TGGTATC6600.042.9302982
CCTGGCG300.001920422136.66599732
GTTGTAT350.004075214331.4491211
CAACCCA350.004088649531.4279945
ATAAACG350.004088649531.4279943
GTAAGCC350.004088649531.42799435
GTATCAA23450.027.8113521
CTGCTTA400.00785220427.49949618
GCTGGTA606.282254E-527.4994967
GATCACG400.00785220427.49949622
GGTATCC606.282254E-527.49949610
GGTATCA11450.025.9383873
GAATAAG550.001216893325.016351
GGGGCAA1101.173612E-824.99954418
ACACATA550.00122167424.99954455
CTGGTAT550.00122167424.9995448
CGGGGCA1101.173612E-824.99954417
Produced by FastQC (version 0.11.5)