FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57550
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA3920.6811468288444831No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3090.5369244135534318No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT2480.43092962641181587No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2160.37532580364900087No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA1960.34057341442224154No Hit
GAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA1950.3388357949609036No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA1920.33362293657688963No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1780.30929626411815814No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT1640.2849695916594266No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA1430.24847958297132927No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAA1290.22415291051259773No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.20851433536055605No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.16507384882710688No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.15464813205907907No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA770.13379669852302345No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770.13379669852302345No Hit
ATTGATAACCTTGTTTTGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.13032145960034752No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.12858384013900956No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAT150.001024563785.000016
AGCCAAT200.00320793963.7500041
GCGTCAG200.00320793963.7500044
GTGGTAT1200.053.1250041
AGCGTCA250.00775889651.03
AAGGTTA250.00775889651.05
CTCCATT250.00775889651.06
GGATATA250.00775889651.01
CGTCAGA250.00775889651.05
ACAAGGT250.00775889651.03
TGGTATC1350.047.222222
GTATCAA4400.043.4659081
GGGATAC1850.039.0540547
ACGGGAT1900.038.0263145
TATCAAC5350.036.5420532
CGGGATA2050.035.2439046
TCGAGGG1600.034.5312549
GGATACC2100.034.4047628
GGTATCA2100.034.4047623
CAGTGGT500.002767136834.01
Produced by FastQC (version 0.11.5)