FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1557242
Sequences flagged as poor quality0
Sequence length91
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA127440.8183699129615051No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT75180.4827766011962174No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69390.4455954822692941No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA66680.4281929205608377No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54970.352995873473744No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT51530.33090553684013146No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48800.3133745429419448No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA47230.30329261604811586No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT27270.1751172906972712No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA24750.15893483479125273No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24670.15842110603233153No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA23450.1505867424587829No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA22920.14718328943092981No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC21690.13928470976251603No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20000.12843218973030526No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATC19970.1282395414457098No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19060.12239587681298089No Hit
GTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTC17300.11109384411671402No Hit
GCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTA17130.11000217050400644No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17110.10987373831427615No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16630.10679136576074882No Hit
TATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAA15750.10114034941261538No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT38550.064.281031
TGGTATC42150.059.793492
CGTATCA802.1282176E-1047.8179741
GGTATCA89400.040.9358941
GCAGCGT4100.039.3947531
GTATCAA174650.039.2801931
CAGCGTC4750.034.895372
TATCAAC206350.033.695792
CGACTTT14950.033.5457573
CCCTTGA7700.032.016651
GATATAC95300.031.88981
GGTTATC4400.031.8776237
ATATACG99500.031.3950442
AGCGTCA5300.031.274153
TCGACTT15850.031.1046662
TGTATAA16750.030.9557842
GCATCGT1101.861481E-730.9116348
ATACGCA101250.030.8524114
CAAGGTT4850.030.6706584
GCGTCAG5450.030.4133954
Produced by FastQC (version 0.11.5)