FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1742763
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG97480.5593416890305796No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39920.22906155340686024No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21720.12462968286565644No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA17460.10018573954117685No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT61300.046.3243561
TGGTATC64700.043.87742
TTAAACG1600.032.657822
AGCGGTA507.034974E-427.4989428
CCTTGAT8450.026.3690092
CGCGTTA759.756952E-625.66641448
GTATCAA218050.025.4656751
GGTATCA115450.025.3440533
CGACGGG1001.311364E-724.74833510
GCGTTAT801.6089743E-524.06226249
GTTATCG801.6096894E-524.0608851
AGAGTAC112250.023.71332611
CAGAGTA117200.022.94640710
GACGTAT600.002035387622.91775520
TATCAAC250200.022.1760442
CCCTTGA8300.021.8804361
TATCCCG3450.021.52028812
CCGACGA5050.021.23802255
ATCGACG650.003240599821.1536353
TGTTATC3000.021.089782
Produced by FastQC (version 0.11.5)