FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1145738
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA83720.7307080676385003No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT49100.4285447458319441No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48910.4268864260415558No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA44540.38874507086262305No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA42650.37224915294770705No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36000.3142079602841138No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT33000.288023963593771No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31520.2751065252265352No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA21390.1866918964021443No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA19510.17028325847619613No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT18090.1578895000427672No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA17740.15483470042889386No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC14680.1281270238047442No Hit
GTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTC14070.12280294447770783No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATC13920.12149374464319068No Hit
GCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTA13830.12070822474248039No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13490.11774070511757487No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAA13240.11555870539337965No Hit
GAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA12350.1077907863752446No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCCTGTCTCTTA12010.10482326675033907No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACCTGTC11760.10264126702614386No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT26250.060.398291
TGGTATC29500.053.7372062
GCGTCAG2900.045.4309654
GTATCAA117900.041.6402441
GGTATCA59400.039.857851
TATCAAC136450.036.441862
CGTCAGA3950.034.430335
AGCGTCA3950.033.3543783
GACAGGT5150.033.013981
ATCAACG156900.031.7462843
CCCTTGA4750.031.3198411
CAGTACG952.0723091E-631.3198411
TCAACGC159350.031.2315184
GATCTAC1101.8599894E-730.913091
GTCGACC550.00444803630.9090428
CAACGCA161350.030.765375
CCTTGAT7500.029.4666232
AACGCAG169050.029.3891896
TCGAGGG44050.027.8830439
CGACTTT10450.027.655463
Produced by FastQC (version 0.11.5)