FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1329287
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA69340.5216330258251227No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT48080.3616976619796929No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45600.3430410438076954No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA40600.3054268942673779No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34740.26134311100612584No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA34150.25690464136036834No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT30910.23253067245824266No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30700.23095087817754933No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA20640.15527120930243055No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT18120.13631367793411053No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA17650.1327779478773207No Hit
GAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA15970.12013959363177402No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAA14280.10742601108714672No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13680.10291231314230863No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT25850.056.8971861
TGGTATC28050.053.1843872
AGCGTCA3600.050.766343
GCGTCAG4300.043.490474
GGTATCA53900.041.0100061
GTATCAA106500.039.953951
TATCAAC123600.034.730642
ATCAACG138250.031.1118073
TCAACGC140700.030.5096444
CAACGCA141800.030.3029445
CGTCAGA6100.029.960465
AACGCAG145950.029.4726356
TCGAGGG38450.026.197629
GATATAC66300.026.0949291
GCAGCGT6850.026.0635741
ATATACG67600.026.0293622
CGCATTG67950.025.5825447
ATACGCA68500.025.5632784
CAGCGTC7150.025.5606732
ACGCATT68600.025.4040136
Produced by FastQC (version 0.11.5)