FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415886
Sequences flagged as poor quality0
Sequence length61
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG203164.884992521989199No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32820.789158567492053No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16370.3936174817137389No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA12590.30272718966255174No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10260.24670222128179356No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCCTGTCTCTTATACACATCTGA10200.245259518233362No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATCTGACGCTGCCG10120.24333591416878664No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACCTGTCTCTTATACACA8680.20871104100642965No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCG8040.19332220848982654No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAA7970.1916390549333231No Hit
GCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGA7520.1808187820700865No Hit
GTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACA6680.16062093939204494No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCTGTCTCTTATACA5880.14138489874629107No Hit
ATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACA4760.11445444184223562No Hit
CTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT4600.11060723371308484No Hit
GGTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAA4590.1103667832050129No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGCTGTCTCTTA4430.10651957507586214No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCCTGTCTCTTATACACATCTG4280.10291281745478328No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATCTGACGCTGCC4260.10243191643863944No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAC150.00578142654.9990411
ATATCCG202.6119102E-454.9990425
TATCTGC150.00578142654.9990414
TACGACT150.00578142654.9990428
CGTACGG150.00578142654.9990413
GCGTACG150.00578142654.9990412
AGTGTCG150.00578142654.9990411
AGTGTAC150.00578142654.9990422
TACAATC150.00578142654.9990422
TATCCGC202.6119102E-454.9990426
ACGCATC453.265086E-948.88803529
CGCATCT453.265086E-948.88803530
GTGGTAT23050.044.65721
CTGCTTA508.290954E-943.9992318
TCCGCAT257.8513555E-443.9992328
TGCTTAA508.290954E-943.9992319
ATCCGCA257.8513555E-443.9992327
GCCTCGT257.8513555E-443.9992314
CGCAGTG257.8513555E-443.999238
CCGCATT257.8513555E-443.9992329
Produced by FastQC (version 0.11.5)